Microscope objectives
An introduction to 'infinity' corrected microscope objectives.
ContentsTransmission
The many elements that usually make up an objective lens reflect and absorb the collected light, typically reducing the overall transmission by 10-20%. So it is important to check the transmittance curve for the intended wavelength range, for example here is the data for a Nikon 100x1.35 Silicone objective (MRD73950):
